Next generation sequencing, bioinformatics Next generation sequencing, RNAseq, gene expression profiling, downstream effects of fusion genes, alternative splicing
Role of host factors in HIV infection and the biology of HIV in animal models Gateway cloning system for assembling coding regions, 3'UTRs, and mRNA tags (under development with Dom Esposito, ATP, ATRF)ĭirect assessment of transcription fidelity by high-resolution RNA sequencing Mechanisms of transcription through chromatin by RNA polymerase II and III Determinants of RNA polymerase processivity in transcription elongation DNA-protein and DNA-RNA interactions in elongation complex of RNA polymerase Translating deranged microRNA profiles unique to mesothelioma and other thoracic cancers for biomarker discovery and novel therapeutic strategiesģ'UTR-mediated regulation of mRNA localization and protein activity signaling transcription factors Profiles of miRNAs and inflammation genes as they relate to cancer diagnosis, prognosis and therapeutic outcome RNA-induced silencing complex assembly Novel design of potent DNA-directed RNAi (shRNA) with minimal off-target effects Fidelity Of miRNA Biogenesis - Dissecting Dicer Processing In Vivoġ) bioinformatics, 2) transcriptional regulation, 3) Single cell omics, 4) cancer genomics RNAi role in targeting of heterochromatin complexes to specific locations in the genome Laboratory of Biochemistry and Molecular Biology Small Regulatory RNAs and Energy-Dependent Proteolysis: Novel Modes for the Regulation of Gene Expression HIV/retrovirus assembly, viral protein trafficking, retrovirus replication, HIV-1 maturationġ) alternative splicing, 2) RNA biology, 3) gene regulation, 4) genome engineering, 5) CRISPR-Cas, 6) functional genomics HIV DRP Retroviral Replication Laboratory
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